How TPAcykTM ELISA Works
TPAcykTM ELISA is a one-step, enzyme-linked sandwich immunoassay. Standards, controls, and samples react simultaneously with solid phase catcher antibodies and HRP-conjugated detector antibodies. After washing, a substrate is added and a color reaction will develop, which is proportional to the amount of cytokeratin. The concentration is determined by comparison with a standard curve with known concentrations of cytokeratin.

How TPAcykTM IRMA Works
TPAcykTM IRMA is a one-step, solid phase immunoradiometric sandwich assay. Standards, controls, and samples react simultaneously during incubation with solid phase catcher antibodies coated on beads and detector antibody. After washing, samples are assessed for radioactivity, which is proportional to the amount of cytokeratin. The concentration is determined by comparison with a standard curve with known concentrations of cytokeratin.